* The cost of DNA origami is from 1,000$ to 10,000$ per nmol. The scaffold strand dominates the total cost (80%).
* The method cannot be scaled up arbitrarily
– requires scaffolds of larger size.
– sequences of staple strands should be unique.
Design of Scaffolded DNA Origami
a. the target shape
b. discrete approximation
c. hand-engineered folding path
d. staple strand geometry
e. generated staple strand DNA sequences
[synthesize the staple strands]
[mix the scaffold and staple strands]
f. the resulting shape as seen from atomic force microscope