Scientists have for the first time reengineered a building block of a geometric nanocompartment that occurs naturally in bacteria. They introduced a metal binding site to its shell that will allow electrons to be transferred to and from the compartment. This provides an entirely new functionality, greatly expanding the potential of nanocompartments to serve as custom-made chemical factories.
Scientists hope to tailor this new use to produce high-value chemical products, such as medicines, on demand.
The sturdy nanocompartments, which are polyhedral shells composed of triangle-shaped sides and resemble 20-sided dice, are formed by hundreds of copies of just three different types of proteins. Their natural counterparts, known as bacterial microcompartments or BMCs, encase a wide variety of enzymes that carry out highly specialized chemistry in bacteria.
The shell of a bacterial microcompartment (or BMC) is mainly composed of hexagonal proteins, with pentagonal proteins capping the vertices, similar to a soccer ball (left). Scientists have engineered one of these hexagonal proteins, normally devoid of any metal center, to bind an iron-sulfur cluster (orange and yellow sticks, upper right). This cluster can serve as an electron relay to transfer electrons across the shell. Introducing this new functionality in the shell of a BMC greatly expands their possibilities as custom-made bio-nanoreactors. (Credit: Clément Aussignargues/MSU, Cheryl Kerfeld and Markus Sutter/Berkeley Lab)
Researchers at the Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) devised synthetic shell structures derived from those found in a rod-shaped, ocean-dwelling bacterium, Haliangium ochraceum, and reengineered one of the shell proteins to serve as a scaffold for an iron-sulfur cluster found in many forms of life. The cluster is known as a “cofactor” because it can serve as a helper molecule in biochemical reactions.
BMC-based shells are tiny, durable and naturally self-assemble and self-repair, which makes them better-suited for a range of applications than completely synthetic nanostructures.
“This is the first time anyone has introduced functionality into a shell. We thought the most important functionality to introduce was the ability to transfer electrons into or out of the shell,” said Cheryl Kerfeld, a structural biologist at Berkeley Lab and corresponding author in this study. Kerfeld’s research group focuses on BMCs. Kerfeld holds joint appointments with Berkeley Lab’s Molecular Biophysics and Integrated Bioimaging (MBIB) Division, UC Berkeley and the MSU-DOE Plant Research Laboratory at Michigan State University (MSU).
“That greatly enhances the versatility of the types of chemistries you can encapsulate in the shell and the spectrum of products to be produced,” she said. “Typically, the shells are thought of as simply passive barriers.”
Enzymes inside natural BMCs can convert carbon dioxide into organic compounds that can be used by the bacteria, isolate toxic or volatile compounds from the surrounding cell, and carry out other chemical reactions that provide energy for the cell.
In this study, researchers introduced the iron-sulfur cluster into the tiny pores in the shell building block. This engineered protein serves as an electron relay across the shell, which is key to controlling the chemical reactivity of substances inside the shell.
Clément Aussignargues, the lead author of the study and postdoctoral researcher in the MSU-DOE Plant Research Laboratory in Michigan, said, “The beauty of our system is that we now have all the tools, notably the crystallographic structure of the engineered protein, to modify the redox potential of the system—its ability to take in electrons (reduction) or give off electrons (oxidation).
“If we can control this, we can expand the range of chemical reactions we can encapsulate in the shell. The limit of these applications will be what we put inside the shells, not the shells themselves.”
He added, “Creating a new microcompartment from scratch would be very, very complicated. That’s why we’re taking what nature put before us and trying to add to what nature can do.”
To design the metal binding site, Kerfeld’s group first had to solve the structures of the building blocks of the nanocompartment to use as the template for design. These building blocks self-assemble into synthetic shells, which measure just 40 nanometers, or billionths of a meter, in diameter. The natural form of the shells can be up to 12 times larger.
The iron-sulfur cofactor of the engineered protein, which was produced in E. coli bacteria, was very stable even when put through several redox cycles—a characteristic essential for future applications, Aussignargues said. “The engineered protein was also more stable than its natural counterpart, which was a big surprise,” he said. “You can treat it with things that normally make proteins fall apart and unwind.”
A major challenge in the study was to prepare the engineered protein in an oxygen-free environment to form tiny crystals that best preserve their structure and their cofactor for X-ray imaging, Kerfeld said. The crystals were prepared in an air-sealed glovebox at MSU, frozen, and then shipped out for X-ray studies at Berkeley Lab’s ALS and SLAC National Accelerator Laboratory’s Stanford Synchrotron Radiation Lightsource (SSRL).
Bacterial microcompartments (BMCs) are self-assembling organelles composed of a selectively permeable protein shell and encapsulated enzymes. They are considered promising templates for the engineering of designed bionanoreactors for biotechnology. In particular, encapsulation of oxidoreductive reactions requiring electron transfer between the lumen of the BMC and the cytosol relies on the ability to conduct electrons across the shell. We determined the crystal structure of a component protein of a synthetic BMC shell, which informed the rational design of a [4Fe-4S] cluster-binding site in its pore. We also solved the structure of the [4Fe-4S] cluster-bound, engineered protein to 1.8 Å resolution, providing the first structure of a BMC shell protein containing a metal center. The [4Fe-4S] cluster was characterized by optical and EPR spectroscopies; it has a reduction potential of −370 mV vs the standard hydrogen electrode (SHE) and is stable through redox cycling. This remarkable stability may be attributable to the hydrogen-bonding network provided by the main chain of the protein scaffold. The properties of the [4Fe-4S] cluster resemble those in low-potential bacterial ferredoxins, while its ligation to three cysteine residues is reminiscent of enzymes such as aconitase and radical S-adenosymethionine (SAM) enzymes. This engineered shell protein provides the foundation for conferring electron-transfer functionality to BMC shells.
SOURCES- Journal of American Chemical Society, Lawrence Berkeley National Lab