Construction of integrated gene logic-chip

In synthetic biology, the control of gene expression requires a multistep processing of biological signals. The key steps are sensing the environment, computing information and outputting products. To achieve such functions, the laborious, combinational networking of enzymes and substrate-genes is required, and to resolve problems, sophisticated design automation tools have been introduced. However, the complexity of genetic circuits remains low because it is difficult to completely avoid crosstalk between the circuits. Here, we have made an orthogonal self-contained device by integrating an actuator and sensors onto a DNA origami-based nanochip that contains an enzyme, T7 RNA polymerase (RNAP) and multiple target-gene substrates. This gene nanochip orthogonally transcribes its own genes, and the nano-layout ability of DNA origami allows us to rationally design gene expression levels by controlling the intermolecular distances between the enzyme and the target genes. We further integrated reprogrammable logic gates so that the nanochip responds to water-in-oil droplets and computes their small RNA (miRNA) profiles, which demonstrates that the nanochip can function as a gene logic-chip. Our approach to component integration on a nanochip may provide a basis for large-scale, integrated genetic circuits.

Nature Nanotechnology – Construction of integrated gene logic-chip

6 thoughts on “Construction of integrated gene logic-chip”

  1. Unless Zuck sells it, or, other investors sell it for less than they purchased it for, it’s not that big of a deal “to them”.It’s just paper until you convert it to cash.

    Reply
  2. I think vukkle sucks. This comment was judged to be “85% likely to be perceived as “toxic” ” . Is that because I used the word “vukkle”. Now “78% likely to be perceived as “toxic”

    Reply
  3. How much does FB actually earn? I don’t really do FB, I just log into my late wife’s acct. to check if my in laws put up anything about my grandson. I have never seen any ads, or maybe I am just so immune to ads that I do not even take cognizance of them.

    Reply
  4. Finally. I have always wondered how could FB have so high market cap. I do not know you, but I do not recall have ever clicked on one Facebook ad ever I believe FB is just a big bubble

    Reply
  5. Companies that have share prices as inflated as Facebook are subject the great price swings when the growth rate of their revenues change.

    Reply

Leave a Comment