For DNA-based target recognition systems, the two major approaches, multiplex automated genome engineering (MAGE) using λ-red recombination and conjugative assembly genome engineering (CAGE) have been widely used in prokaryotes and demonstrated a high degree of multiplexability. Briefly, the λ-red system, similar to recET, originates from phage and when imported into bacterial cells, can stimulate high levels of recombination in the presence of homologous DNA. Although the machinery used in these approaches has not been fully optimized yet for eukaryotic cells, some conjugal transfer has been demonstrated and use of an optimized version of Redβ in human cells has yielded limited success. Moreover, these technologies can be used to edit and propagate large pieces of DNA in bacteria for eventual use as donor DNA molecules for HR in human cells.
Brian Wang is a prolific business-oriented writer of emerging and disruptive technologies. He is known for insightful articles that combine business and technical analysis that catches the attention of the general public and is also useful for those in the industries. He is the sole author and writer of nextbigfuture.com, the top online science blog. He is also involved in angel investing and raising funds for breakthrough technology startup companies.
He gave the recent keynote presentation at Monte Jade event with a talk entitled the Future for You. He gave an annual update on molecular nanotechnology at Singularity University on nanotechnology, gave a TEDX talk on energy, and advises USC ASTE 527 (advanced space projects program). He has been interviewed for radio, professional organizations. podcasts and corporate events. He was recently interviewed by the radio program Steel on Steel on satellites and high altitude balloons that will track all movement in many parts of the USA.
He fundraises for various high impact technology companies and has worked in computer technology, insurance, healthcare and with corporate finance.
He has substantial familiarity with a broad range of breakthrough technologies like age reversal and antiaging, quantum computers, artificial intelligence, ocean tech, agtech, nuclear fission, advanced nuclear fission, space propulsion, satellites, imaging, molecular nanotechnology, biotechnology, medicine, blockchain, crypto and many other areas.