CRISPR-Cas9 has been use to stop HIV in infected T cells

Scientists in Japan have used CRISPR-Cas9 technology to stop human immunodeficiency virus type 1 (HIV-1) replication in latently infected T cells that can’t be controlled using existing drug treatments. The gene-editing approach effectively disrupts two regulatory HIV-1 genes, tat and rev, which are essential for viral replication. Describing their in vitro studies in Scientific Reports, the researchers at Kobe University Graduate School of Medicine and Kobe University Graduate School of Health Sciences say initial results indicate that using CRISPR-Cas9 to target HIV-1 regulatory genes may offer a new approach to achieving “functional cures.”

“These results show that the CRISPR-Cas9 system, by targeting the regulatory genes of HIV-1, tat and rev, is a promising method for treating HIV infection,” comments co-author Masanori Kameoka, Ph.D., an associate professor at Kobe University Graduate School of Health Sciences. “We now need to investigate how we can selectively introduce a CRISPR-Cas9 system that targets HIV-1 genes into the infected cells of patients.”

HIV-1 infects about 35 million people worldwide, and while lifelong antiretroviral therapy (ART) can help convert what is otherwise a deadly infection into a more “manageable chronic disease,” current treatments are not a cure because they can’t completely eradicate the virus, which inserts its genes into the host cells’ DNA, the authors explain. Despite treatment using ART, HIV-1 continues to replicate at a very low level in some latently infected immune system cell types, such as CD4+ cells, macrophages, and follicular dendritic cells. “Current antiviral compounds are incapable of targeting the integrated proviral genome inside these cellular reservoirs and rapid viral rebound ensues after ART cessation,” they explain. The virus can also hide out in tissues such as the central nervous system.

Nature Scientific Reports – CRISPR/Cas9 System Targeting Regulatory Genes of HIV-1 Inhibits Viral Replication in Infected T-Cell Cultures.

In conclusion, the results of the present study showed that the CRISPR/Cas9 system is robust and efficient for targeting the HIV-1 proviral genome to suppress replication in latency models. CRISPR efficiency mainly depends on how well the gRNA sequence matches the target DNA, and targeting of the highly conserved regulatory genes tat and rev is beneficial for this effect. Moreover, the importance of these genes to the transcription ability of HIV-1 may lessen the emergence of CRISPR escape mutants. The combination of multiple gRNAs may also maximize efficiency and minimize the risk of resistance. Recent studies may have proven efficacy in cell cultures and ex vivo; however, when in vivo safety and efficacy profiles have been established, CRISPR-bearing lentiviral vectors may be delivered into HIV-1-infected individuals to clear latent viral reservoirs. Based on the rapid advances being achieved in CRISPR/Cas9 research, a HIV-1 functional cure may soon be within reach.

15 thoughts on “CRISPR-Cas9 has been use to stop HIV in infected T cells”

  1. Ellesandra Ryan is my name. I want you to understand and believe that at the end of every difficulty there surely will be a relief for no condition is absolutely permanent and no illness without cure except you have not used my herbs, be hopeful, You didn’t come with this from god and no human deserves to be sick even if it s your making out of human error. These were the words of Dr White when I first wrote him on watsapp using 2349091844595. You can also reach him via email: drwhitethehivhealer@gmail.com. I was having the biggest nightmare ever (HIV) Medical doctors kept saying no cure, calm down and we can manage it but the thought of using ART for ever and its devastating side effects will not let me. Hope was lost and dreams shattered but Dr White, a non scientist and unpopular restored them all after reading about him in a blog article published by Jose where he was generally acknowledged to being able to cure HIV/AIDS and other viruses using ancient herbal remedies. I know exactly how you feel reading this but if you give up now it will amount to suicide. Be assure with me as witness that Dr White’s miracle herbal cleanser which he sends through trusted courier lines will get to you anywhere and you will be victorious. You may have tried other means without success but just this once your prayer is answered. I wrote this to save you and keep a promised made.
    Ellesandraryan01@outlook.com

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  2. Popular saying health is wealth, I suffered from HSV-2[Herpes Simplex
    Viruses, HSV-2] Herpes, typically appear as blister on my mouth ,genitals
    and Rectum.
    Sandra Decrion, Am from Lyon, France , french citizenship, we lives in
    EDMONTON Canada with my families.
    I contacted Herpes 2013, them my body change rapidly , leading to fatigue ,
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    Reply
  3. Popular saying health is wealth, I suffered from HSV-2[Herpes Simplex
    Viruses, HSV-2] Herpes, typically appear as blister on my mouth ,genitals
    and Rectum.
    Sandra Decrion, Am from Lyon, France , french citizenship, we lives in
    EDMONTON Canada with my families.
    I contacted Herpes 2013, them my body change rapidly , leading to fatigue ,
    general illness[ from mild illness to serious conditions]
    Immune-Suppression due to chemotherapy or steroid and lastly Trauma of the
    affected area.
    Dr.ISE Herbal Therapeutic medicine are awesome, powerful, highly effective,
    curative, they are pure, and natural medications.All medication was
    shipped to me In EDMONTON Canada, I used as instructed by Dr.ISE . I
    completed all Herbal medication within a month, it was normal, not bitter,
    1 herbal soap, 1 herbal cream, 1 immunity booster and 2 liquid suspension
    of 1000ml each,total o 5 Herbal . After I completed all Herbal medications
    , I felt something different in my body, I went for medical checkup , it
    was Herpes Negatives complete elimination of Herpes Simplex Viruses”
    HSV-2” from my body system. Today am Herpes free[negative] cured by
    Dr.ISE.
    DR. ISE Registered Email and What-apps Number ‘are;
    ; isespiritualspelltemple@gmail.com
    cureherbal633@gmail.com
    He cure various Illness like HIV, Hepatitis, HPV, Herpes,Asthma, Various
    Skin allergy, infertility in male and female, Cancer,Diabetes, Syphilis,
    Meningitis, ulcer, low sugar,
    High Blood Pressure, Weight loss, Immunity Booster, H

    Reply
  4. Hiv is a serious and recurring disease which can’t be cured through drugs or injections by the American doctors but the best way to deal with HIV is by taking natural herbs medicine for it and is only few American doctors that know about this herbal medicine from Dr Alabi .. I have read about Dr Alabi the great herbalist doctor from African who can cure HIV with his powerful herbal medicine. for the people suffering from the following diseases HIV, Herpes, Cancer, Als,Herpatitis, Diabetes, should contact him for his herbal medicine because i am a living testimony and i was cured of HIV. Although, i sent him what he requested and he sent me his medicine which i took for two weeks and today when i went for test i was tested HIV negative. you can reach him through his WhatsApp number:+2348026903406 or through his gmail dr.alabiherbalhome@gmail.com.

    Reply
  5. Hello readers, i am Gertrude aged 39. Believe it or not HIV/AIDS has a cure but maybe you haven’t meet the right healer. I have waited for this day so that i can share this testimony of how i was cured of my Hiv/Aids by DR WHITE the greatest healer. Never shall I forget these two days so long I lived 1. The day I tested positive to HIV being 09/11/ 2015 and 22/04/2019 being the day Dr White saved me and I tested negative. It will interest you to know that 2different people referred me to this African Dr outside what I read about him on different webs and forums. The day I tested negative will forever remain the happiest day of my existence and Dr White my Idol but thanks to Jehovah because he give the access to what a lot of people believed is impossible. This man only asked a little amount, personal info. and address to send the CURE and within 30days of using the herbals tea I tested good. Write Dr White now on: DRWHITETHEHIVHEALER@GMAIL.COM or Whatsapp: +23490-9184-4595 and be saved forever. An Italia Proverb says A WORD IS ENOUGH FOR THE WISE. I can belt 100% cure for your HIV treatment with Dr White including herpes, Hepatitis, HPV and so on. This so because I have meet directly with two people he cured and I have also referred to him and it turns out real negative. Thanks to God and Dr White forever.
    You may write me on: gertrudethato09@yahoo.com

    Reply
  6. I am really happy that me and my husband are cured of (HIV VIRUS) with the help of Dr Bello herbal medicine, we have been suffering from this disease for the past 2 years without solution until i came across the email of this Herbal Healer who have cured so many people with his herbs, i also choose to give him a chance to help me and my Husband, he told me what to do and i kindly did it, and he gave us his herbal medicine and instructions on how to use it,After using the herbs for 1 weeks we went for a test on 8days of it, to my greatest surprise our result came out as negative, we are really happy that there is someone like Dr Bello who is ready to help anytime any day. to all the readers and viewers that is doubting this testimony stop doubting it and contact this Dr and see if he will not actually help you. i am not a stupid woman that i will come out to the public and start saying what someone have not done for me. he is really a great man contact him now. with this email bellospelltemple@gmail.com he is always ready to help you solve your problem….call or whatsApp him on +2348147271779 or facebookpage http://fb.me/drbelloherbalhome

    Reply
  7. I believe it’s a shame on me if i don’t share this story of how I escaped death as a result of HIV. I was never sick but only went for routine medical checks when the news of being HIV was broken to me and instantly I went blank and speechless for more than 30 minutes because to me HIV is death sentence. I went into search for cure and prayer section without solution even as I refused ARV drugs for about 3year 9months until I began to feel sick and down with diarrhea that I rushed to hospital and started using ARV. A confidant showed me a magazine where doctor White was said to cure HIV I extracted his number and wrote him watsapp: (+2349091844595) Haven written doc. WHITE he asked I keep calm, pay for the medicines, use the medicines exactly as prescribed by him and he will guarantee me a cure. he also requested some of my personal information and address to enable him fill a medical form for me and send the medicines after preparation. Although i didn’t believe him at first, i was just trying to out of frustration. 4 weeks exactly, i went for new tests, and you won’t believe that 8 different doctors have confirmed it that I’m HIV NEGATIVE. It was like a dream, I never believe HIV has cure.He also cures Cancer, Herpes, Hepatitis Genital Warts and more. A man by name Jose have published an article on national British daily and grant live interview to BBC on how he was cured by this doc. Remember PROPHETS are usually not honored in their life time. You can also write him on em

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  8. Greetings to the general public, I’m Wenche Klopp from Stavanger Norway. I have been living with Hiv virus for 7years and I was cheated three times and many false Doctors took advantage of this Disease to deceive me and took my hard earned money, As a result of my experience I do not have to blame some people finding it difficult to believe there is a cure for hiv Virus. I have been HIV-positive for years but today, I’m happy to be HIV-negative using Dr. APAMA ‘s herbal remedies. I have been going to places and doing research looking for help on the Internet when I came across people sharing testimonies about how Dr. APAMA cured them of HIV, HERPES, CANCERS, HEPITIS, HPV, TUMOR And many other diseases medically incurable. I always thought there is no solution because I have tried so many things but didn’t work. I talked to Dr. APAMA as I explained my health status to him. Fortunately for me, I received the medicine and used it according to the instructions after meeting the requirements. I’m very glad and I want to share my experience to everyone for the benefit of those going through one Disease and the other to please quickly contact this great Man by whatsapp: +2349072570496 OR at Email: DRAPAMAHERBALHEALINGCENTRE@GMAIL.COM. I have also referred to Dr. APAMA those looking for the following cure: Hpv, herpes, Vaginal infections, hepatitis B, Cancers, hepatitis C, infertility problem, diabetes, Kidney problem, asthma and more. My Email is Klopwenche@gmail.com

    Reply
  9. AN AMAZING REMEDY FOR HIV. It’s so unbelievable how wonderful herbs can be. when I first saw a post that said that a man have a perfect cure for HIV, I was like could this be real or some kind of scam but due to my eagerness to be cured I took the risk of contacting him, he gave me reasons to believe he is a real herbalist so I followed all his instructions and now all my test results shows HIV(-) negative , Dr. Zanbo have the most safe cure for herpes and other stubborn viruses. contact him via his WhatsApp: +2347059420361 and email zanbospiritualhome@gmail.com trust me you will like this method, it’s safe cheap and easy. do well by letting others know about this

    Reply
  10. AN AMAZING REMEDY FOR HIV.It’s so unbelievable how wonderful herbs can be. when I first saw a post that said that a man have a perfect cure for HIV I was like could this be real or some kind of scam but due to my eagerness to be cured I took the risk of contacting him he gave me reasons to believe he is a real herbalist so I followed all his instructions and now all my test results shows HIV(-) negative Dr. Zanbo have the most safe cure for herpes and other stubborn viruses.contact him via his WhatsApp: +2347059420361 and email zanbospiritualhome@gmail.com trust me you will like this method it’s safe cheap and easy. do well by letting others know about this”

    Reply
  11. I have been surffering from HIV Virus for the past 4years.I eventually got married to my husband who was also HIV positive 2years back.i and my husband have ever since then submitted ourselves to taking medications and eating fruits as directed by our family doctor.i was surfing the web recently to check out some herbal remedies.i saw many comments and many posts until i saw a testimony about Dr ABILI and how he helped so many people get cured of their ailments using herbs, on the site. So I contacted him and told him how i and my husband have been battling with the virus for some time. he told me what we will have to do.He told me that we will take his herbal medication for three weeks(approximately 21days) which we also did.some weeks ago, my husband was the first to go for the test the second time and alas,the result was Negative.I too went for mine after seeing my husband’s result and mine too turned out to be Negative. i and my husband are now HIV negative.If i was told that i will one day be free of this virus, i will strongly doubt it. I am so happy that God used Dr.ABILI to put smiles back on our faces.So at this point, if there’s anybody who is infected and you think there’s no way of making yourself negative again,believe me, all hope ain’t lost yet.Dr ABILI herbal medications will make you negative again. Just talk to Dr. ABILI about whatever ails you. He can cure other sickness like : Herpes, Hemorrhoids, Hepatitis,Diabetes, Cancer, Meningitis, HIV/AIDS, Fibroid, Tuberculosis,Paralysis, among many others… contact Dr.ABILI vis email (drabiliherbalhome@gmail.com) or whats-app mobile: +2349034412845 He is here to help anytime…

    Reply
  12. I have been surffering from HIV Virus for the past 4years.I eventually got married to my husband who was also HIV positive 2years back.i and my husband have ever since then submitted ourselves to taking medications and eating fruits as directed by our family doctor.i was surfing the web recently to check out some herbal remedies.i saw many comments and many posts until i saw a testimony about Dr ABILI and how he helped so many people get cured of their ailments using herbs on the site. So I contacted him and told him how i and my husband have been battling with the virus for some time. he told me what we will have to do.He told me that we will take his herbal medication for three weeks(approximately 21days) which we also did.some weeks ago my husband was the first to go for the test the second time and alasthe result was Negative.I too went for mine after seeing my husband’s result and mine too turned out to be Negative. i and my husband are now HIV negative.If i was told that i will one day be free of this virus i will strongly doubt it. I am so happy that God used Dr.ABILI to put smiles back on our faces.So at this point if there’s anybody who is infected and you think there’s no way of making yourself negative againbelieve me all hope ain’t lost yet.Dr ABILI herbal medications will make you negative again. Just talk to Dr. ABILI about whatever ails you. He can cure other sickness like : Herpes Hemorrhoids HepatitisDiabetes Cancer Meningitis HIV/AIDS Fibroid TuberculosisParalysis among many others… contact Dr.ABILI vis email (drabiliherbalhome@gmail.com) or whats-app mobile: +2349034412845 He is here to help anytime…

    Reply
  13. I am very happy today to share this amazing testimony on how Dr. idahosa the herbal doctor was able to cure me from my HIV Virus with his herbal medicine. I have been a HIV patient for almost 8 months now and have tried different methods of treatment to ensure that I am cured of this terrible disease, but none worked for me, so I had to leave everything to God to handle as I was a Christian who had faith that one day God would intervene in my life, yet I felt so sad and desperate as I was losing almost everything due to my illness, A few months ago while I was surfing the internet I saw different recommendation about Dr idahosa on how he have been using his herbal Medicine to treat and cure people, these people advice we contact Dr. idahosa for any problem that would help immediately, I contacted Dr idahosa and I told him how I got his contact and also about my disease, after some time Dr. idahosa told me to have faith that he would prepare for me a medication of herbal herbs, he told me I would take this medicine for a few weeks and also asked my home for home address so as possible for him to submit the drug for me, so my good friends after all the process and everything Dr idahosa actually sent me the medicine, I took it as I was directed by Dr. idahosa, after a few weeks passed, while on Dr.idahosa medication I began to experience changes in my body, I had to call my doctor at the hospital for some blood test after test my hospital doctor told me that I was no longer with the Hiv virus and my blood is pretty good, I can not even believe this, Friends well today i am Hiv Free and i want everyone to know that there is a cure for Hiv for those who will contact Dr idahosa after reading my testimony, you can kindly contact Dr. idahosa in (dridahosasolutioncenter@gmail.com) or call him +2348134261542, God bless you all …

    Reply
  14. I have been surffering from HIV Virus for the past 4years.I eventually got married to my husband who was also HIV positive 2years back.i and my husband have ever since then submitted ourselves to taking medications and eating fruits as directed by our family doctor.i was surfing the web recently to check out some herbal remedies.i saw many comments and many posts until i saw a testimony about Dr ABILI and how he helped so many people get cured of their ailments using herbs, on the site. So I contacted him and told him how i and my husband have been battling with the virus for some time. he told me what we will have to do.He told me that we will take his herbal medication for three weeks(approximately 21days) which we also did.some weeks ago, my husband was the first to go for the test the second time and alas,the result was Negative.I too went for mine after seeing my husband’s result and mine too turned out to be Negative. i and my husband are now HIV negative.If i was told that i will one day be free of this virus, i will strongly doubt it. I am so happy that God used Dr.ABILI to put smiles back on our faces.So at this point, if there’s anybody who is infected and you think there’s no way of making yourself negative again,believe me, all hope ain’t lost yet.Dr ABILI herbal medications will make you negative again. Just talk to Dr. ABILI about whatever ails you.
    He can cure other sickness like : Herpes, Hemorrhoids, Hepatitis,Diabetes, Cancer, Meningitis, HIV/AIDS, Fibroid, Tuberculosis,Paralysis, among many others… contact Dr.ABILI vis email (drabiliherbalhome@gmail.com) or whats-app mobile: +2349034412845 He is here to help anytime…

    Reply
  15. AN AMAZING REMEDY FOR HIV.
    It’s so unbelievable how wonderful herbs can be. when I first saw a post that said that a man have a perfect cure for HIV, I was like could this be real or some kind of scam but due to my eagerness to be cured I took the risk of contacting him, he gave me reasons to believe he is a real herbalist so I followed all his instructions and now all my test results shows HIV(-) negative , Dr. Zanbo have the most safe cure for herpes and other stubborn viruses.
    contact him via his WhatsApp: +2347059420361 and email zanbospiritualhome@gmail.com trust me you will like this method, it’s safe cheap and easy. do well by letting others know about this

    Reply

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