RNA Guided Human Gene and Genome Engineering and Radical life extension, disease prevention and cures
Science – RNA-Guided Human Genome Engineering via Cas9 Bacteria and archaea have evolved adaptive immune defenses termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems that use short RNA to direct degradation of foreign nucleic acids. Here, we engineer the type II bacterial CRISPR system to function with custom guide RNA (gRNA) in human …